Protein Extraction From Leaves of Aloe vera L., A Succulent and Recalcitrant Plant, for Proteomic Analysis |
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Authors: | Cong-Fen He Yang-Meng Wang |
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Institution: | (1) Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing, 100037, China |
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Abstract: | An improved method for extracting proteins from leaf tissues of Aloe vera L., a recalcitrant plant species, for proteomic analysis is presented. In this protocol, the following critical components
are included. A washing step is added prior to homogenization of the tissue to eliminate contaminants, and a concentrated
2× extraction buffer (pH 7.5) is used to increase protein yield. Compared to classical trichloroacetic acid–acetone and phenol
extraction methods, this novel protocol has yielded two-dimensional electrophoresis gels with minimal (if any) streaking and
provided high-quality protein samples. This protocol is expected to be applicable to other recalcitrant plant tissues. |
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Keywords: | Aloe vera L Plant protein extraction Proteomics Two-dimensional electrophoresis |
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