Purification and characterization of hatching enzyme of the pike (Esox lucius) |
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| Affiliation: | 1. Sustainable Soils and Grassland Systems Department, Rothamsted Research, North Wyke, Okehampton EX20 2SB, UK;2. Centre for Rural Policy Research, University of Exeter, Exeter EX4 4SB, UK;3. School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK;4. School of Geographical Sciences, University of Bristol, University Road, Bristol BS8 1SS, UK;5. Eden Rivers Trust, Newton Rigg College, Newton Rigg, Penrith, Cumbria CA11 0AH, UK;6. School of Environmental Science, University of East Anglia, Norwich NR4 7TJ, UK;7. Farm Systems and Environment Ltd, Low Road, Wortwell, Norfolk IP20 0HJ, UK |
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| Abstract: | - 1.1. A choriolytic enzyme was isolated from the hatching medium of the pike, Esox lucius.
- 2.2. The enzyme is defined as hatching enzyme.
- 3.3. The molecular weight of the enzyme is 24,000.
- 4.4. The enzyme is a glycoprotein containing 2% carbohydrate.
- 5.5. Its isoelectric point is 6.5.
- 6.6. The pH optimum is around pH 8.
- 7.7. The enzyme molecule contains two disulfide bonds but no free cysteine.
- 8.8. Inhibitor studies and metal analysis show that the enzyme is a zinc-metalloprotease.
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