Hormonal control of testicular protein synthesis in developing Tenebrio molitor |
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| Affiliation: | 1. Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Egypt;2. Department of Pharmacology, Central Laboratory, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Egypt;3. Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, PO Box 35516, Mansoura, Dakahlia, Egypt;4. Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia;5. Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Egypt;6. Department of Animal Histology and Anatomy, Faculty of Veterinary Medicine, Badr University in Cairo (BUC), Cairo, Egypt;7. Department of Biochemistry and Animal Physiology, Faculty of Veterinary Medicine, Badr University in Cairo (BUC), Cairo, Egypt;8. Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Egypt;9. Laboratory of Biotechnology, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Egypt;10. Aquaculture Nutrition Research Unit ANRU, Carna Research Station, Ryan Institute, College of Science and Engineering, University of Galway, Galway H91V8Y1, Ireland |
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| Abstract: | During differentiation, the testes of Tenebrio molitor were found to exhibit increases in biosynthetic capacity reflected by changes in the testicular protein content. A gradual increase in testicular protein content was observed during the prepupal stage. The observed increase was more dramatic in the pupal stage and reached its maximum level between days 4 and 7 of pupal development. During the adult stage, the biosynthetic processes for producing protein were apparently reduced following the first few days after adult emergence.The incorporation of radioactive leucine into TCA-precipitable testicular protein was not affected by the administration of exogenous juvenile hormone alone (JH I, 1 μg/animal) during the pupal stage. However, the administration of exogenous ecdysterone (0.5 μg/animal) to pupal T. molitor resulted in an increase in radioactive leucine incorporated into TCA-precipitable testicular proteins, particularly during the first five days after pupal ecdysis.Simultaneous administration of both JH and ecdysterone to mealworm pupae at specific ages indicated that no apparent interaction, synergistic or antagonistic, occurred between these two hormones with respect to [3H]-leucine incorporation. Furthermore, the amount of [3H]-leucine incorporated approximated to that obtained following injection of ecdysterone alone in all the pupal ages studied. |
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