Calorimetric study of concanavalin A binding to saccharides |
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| Affiliation: | 1. Centro di Studi Chimico-Fisici di Macromolecole, C.N.R., Corso Europa, 30, 16132 Genova Italy;2. Cattedra di Immunologia dell''Università di Genova e Ospedale S. Martino, Viale Benedetto XV, 10, 16132 Genova Italy;1. Institute of Environmental Engineering, National Chiao Tung University, 1001 University Road, Hsinchu 30010, Taiwan;2. Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101 Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan;3. Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan;4. College of Agriculture, Academic Institute, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan;1. Guangxi Key laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China;2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;1. Key Laboratory of Functional Polymer Materials and State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin, 300071, China;2. The Co-Innovation Center of Chemistry and Chemical Engineering of Tianjin, Tianjin, 300071, China;1. Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China;2. Key Laboratory of Detection Technology of Shandong Province for Tumor Marker, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, PR China;1. Centro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, Chapadinha, Maranhão, Brazil;2. Departamento de Patologia, Universidade Federal do Maranhão, São Luís, Maranhão, Brazil;3. Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil;4. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil;5. Curso de Engenharia Química, Universidade Federal do Maranhão, São Luís, Maranhão, Brazil |
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| Abstract: | The binding of concanavalin A in the dimer form to various saccharides has been studied by calorimetry, and estimates of the binding enthalpy and binding constants have been calculated. Methyl α-d-mannoside and methyl α-d-glucoside have a — ΔH0 of 21.5 and 11.5 kJ/mol, respectively, at both pH 4 and 4.5. The p-nitrophenyl derivatives react with enthalpic values of 15.6 and 14.6 kJ/mol. The galactosepyranosides show no heat effects during mixing with the protein solutions.The apparent binding enthalpies calculated from the variations of the equilibrium constants with temperature are in good agreement with the values measured experimentally. The two binding sites of the dimer form of concanavalin A are equal and independent, and the low enthalpies obtained do not justify a large conformational change during the reaction.The binding reaction has also been estimated for other sugars normally contained in glycoproteins. |
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