A characterization of abrin a from the seeds of the Abrus precatorius plant |
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| Affiliation: | 1. Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, CEP 31270-901 Belo Horizonte, MG, Brazil;2. Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, CEP 31270-901 Belo Horizonte, MG, Brazil;3. Centro Universitário Newton Paiva, Avenida Silva Lobo, 1730, CEP 30460-000 Belo Horizonte, MG, Brazil;1. Centre for Genomics and Child Health, Blizard Institute, Queen Mary University of London, London E1 2AT, UK;2. Zvitambo Institute for Maternal and Child Health Research, Harare, Zimbabwe;3. Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA;1. Department of Environmental Microbiology, University of Life Sciences in Lublin, St. Leszczyńskiego 7, 20-069, Poland;2. Department of Environmental Microbiology, Laboratory of Mycology, University of Life Sciences in Lublin, St. Leszczyńskiego 7, 20-069, Poland;3. Department of Vegetable Crops and Medicinal Plants, University of Life Sciences in Lublin, St. Leszczyńskiego 58, 20-068, Lublin, Poland;4. Faculty of Food Sciences and Biotechnology, University of Life Sciences in Lublin, St. Skromna 8, 20-704, Lublin, Poland;5. Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, St. Akademicka 15, 20-950, Lublin, Poland;1. Department of Pharmacy, Shanghai Tenth People''s Hospital, Tongji University School of Medicine, Shanghai 200072, China;2. Department of Neurosurgery, Shanghai Tenth People''s Hospital, Tongji University School of Medicine, Shanghai 200072, China;3. Department of Neurology, Shanghai Tenth People''s Hospital, Tongji University School of Medicine, Shanghai 200072, China |
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| Abstract: | Abrin A was purified from the seeds of the Abrus precatorius plant and its physical and biological properties were studied. The biological properties of abrin A were found to be similar to the better studied Abrus protein, abrin C, in that it is toxic to cell-free protein synthesis and binds d-galactose.Abrin A contains carbohydrate moieties including both neutral and amine sugars but no metals, similar to the other two Abrus proteins (abrin C and the Abrus agglutinin). Amino acid compositions of the subunits of abrin A indicated that it consists of two different subunits of comparable size. Furthermore, one of the subunits showed microheterogeneity suggesting that abrin A is a mixture of isolectins. A comparative study of abrin A and abrin C based on compositions and tryptic maps reveals them to be closely related. The evidence suggests that the two abrins may have the same mechanisms of toxic action.Far-ultraviolet circular dichroic studies of abrin A show it to contain 47% β-pleated sheet and 10% α-helix, again similar to the other two Abrus proteins. |
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