Thymidine catabolism and the reutilization of its degradative products in Tetrahymena pyriformis Metabolism of [2,6-14C2]thymidine and [2-14C]methylmalonic acid |
| |
| Affiliation: | 1. Obstetrical department, The People''s Hospital of Suzhou New District, Suzhou, China;2. Zhejiang Provincial Key Laboratory of Utilization and Innovation of Silkworm and Bee Resources, Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou, China;3. Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, China;1. College of Animal Sciences, Zhejiang University, 310058 Hangzhou, China;2. Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, 310058 Hangzhou, China;3. Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310058 Hangzhou, China;4. Center of Cryo-Electron Microscopy, Zhejiang University School of Medicine, 310058 Hangzhou, China;1. Maastricht University-Aachen Maastricht Institute for Biobased Materials (AMIBM), Urmonderbaan 22, 6167 RD Geleen, the Netherlands;2. Fibrothelium GmbH, Philipsstraße 8, 52068 Aachen, Germany;3. Biomechanics Research Centre (BioMEC), Biomedical Engineering, School of Engineering, College of Science and Engineering, University of Galway, University Road, H91 TK33 Galway, Ireland;4. B4Plastics, IQ Parklaan 2A, 3650 Dilsen-Stokkem, Belgium;5. Umlaut GmbH, Am Kraftversorgungsturm 3, 52070 Aachen, Germany;1. Department of Aerospace, Jain (Deemed-to-be University), Bangalore, India;2. Centre for Research in Functional Materials (CRFM), JAIN (Deemed-to-be University), Jain Global Campus, Bengaluru, 562112, Karnataka, India;3. Interdisciplinary Research Centre (IDRC), JAIN (Deemed-to-be University), Jain Global Campus, Bengaluru, 562112, Karnataka, India;4. School of Agriculture, Food and Wine, Faculty of Sciences, Engineering and Technology, The University of Adelaide, PMB 1 Waite Campus, Glen Osmond, South Australia, Australia;5. Department of Chemical Engineering, NIT Warangal, Telangana, India;6. Amity Institute of Aerospace Engineering, Amity University, Noida, Uttar Pradesh, 201313, India |
| |
| Abstract: | The operation of a reductive pyrimidine catabolic and reutilization pathway in Tetrahymena pyriformis was investigated. Consistent with the proposed catabolic interconversions, radioactivity from [2,6-14C2]thymidine was recovered in respired CO2 30 min after its addition to the culture, whereas, consistent with the proposed anabolic interconversions, over 50% of the incorporated label was recovered in cellular macromolecules other than DNA 12 h after its addition. The chromatographic recovery of 14C radioactivity in monosaccharides from [2,6-14C2]thymidine as well as from [2-14C]methylmalonic acid, a key reutilization intermediate in this proposed pathway, further substantiated the operation of the required anabolic interconversions in this organism. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
