Comparison of nuclear DNA-dependent rna polymerases from mouse tumors and tissues of normal swiss mice |
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| Affiliation: | 1. Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA;2. Wyss Institute for Biologically Inspired Engineering, Harvard University, 60 Oxford Street, Cambridge, MA 02138, USA;3. Kavli Institute for Bionano Science and Technology, Harvard University, 29 Oxford Street, Cambridge, MA 02138, USA;4. Diagnostics for All, 4 Technology Way, Salem, MA 02138, USA;1. Department of Electrical and Computer Engineering, Iowa State University, Ames, IA 50011, USA;2. Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA;1. Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, MA 02138, United States;2. Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr-El Ani Street, Cairo 11562, Egypt;3. Department of Engineering, University of Cambridge, Cambridge CB3 0FA, UK;4. Leeds Vascular Institute, Leeds General Infirmary, Leeds, UK;5. Department of Mechanical Engineering and Division of Surgery, University College London, London, UK;6. Division of Surgery, University of Warwick, Coventry, UK;7. Kavli Institute for Bionano Inspired Science and Technology, School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, MA 02138, United States;8. Wyss Institute for Biologically Inspired Engineering, Harvard University, 60 Oxford Street, MA 02138, United States;1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China;2. Department of Clinical Laboratory, Children''s Hospital of Fudan University, Shanghai, 201102, PR China;3. Shanghai Suchuang Diagnostics Co., Ltd., Shanghai, 201318, PR China;1. Department of Agricultural Economics and Management, School of Public Administration, China Academy for Rural Development (CARD), Zhejiang University, Qizhen Building, Room 1207, Zijingang Campus, China;2. School of Economics and Management, Minjiang University, 200 Xi Yuan Gong Roa, Minhou County, Fuzhou, 350108, Fujian Province, China;3. The York Management School, University of York, Heslington, York, YO105DD, UK;4. School of Humanities and Law, Hangzhou Dianzi University, No. 1158 of Baiyang Street, Xiasha, Hangzhou, China;1. Ministry of Education Key Lab of Water and Sand Science, School of Environment, Beijing Normal University, Beijing 100875, China;2. School of Public Administration, Zhejiang University of Finance and Economics, Hangzhou 310018, China;3. School of Finance, Zhejiang University of Finance & Economics, Hangzhou 310018, China;4. Key Laboratory of Water Cycle and Related Land Surface Processes, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | - 1.1. Five major forms of nuclear DNA-dependent RNA polymerases from seven transplantable murine tumors and from five tissues from normal, adult, male Swiss mice (Mus musculus) were separated chromatographically on DEAE-Sephadex A-25. Forms Ia and Ib were insensitive to α-amanitin, whereas forms IIa and IIb were highly sensitive and form III was slightly sensitive.
- 2.2. The polymerases from all tumors or ascites tumors only were compared statistically with those from the normal tissues in regard to elution patterns, specific activities, activities per mg of nuclear DNA. degrees of purification and yields. Similarly, individual tumors were compared with homologous normal tissues. All parameters were characterized by relatively large variance.
- 3.3. Activities of all RNA polymerases per mg DNA were higher in all tumors compared with normal tissues. The order of statistical significance of these differences was lib > III > IIa > Ib > Ia.
- 4.4. Activities per mg DNA of all RNA polymerases from 6C3HED and L1210 tumors, with the exception of L1210 tumor enzyme III, were significantly greater than those from spleen, but only the activities of Lewis lung tumor enzyme IIb and of Taper hepatoma enzymes Ia and III were higher than those from the homologous tissues, lung and liver, respectively.
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