Sequestration of ascorbic acid by the larval labial gland and haemolymph of the tobacco hornworm,Manduca sexta L. (Lepidoptera: Sphingidae) |
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| Affiliation: | 1. U.S. Grain Marketing Research Laboratory, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Manhattan, KS 66502, U.S.A.;2. Departments of Biochemistry Kansas State University, Manhattan, KS 66506, U.S.A.;3. Departments of Grain Science, Kansas State University, Manhattan, KS 66506, U.S.A.;1. College of Automation, Harbin University of Science and Technology, Harbin, 150080, China;2. College of Shipbuilding Engineering, Harbin Engineering University, Harbin, 150001, China;3. Centre for Marine Technology and Ocean Engineering (CENTEC), Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal;4. International Joint Laboratory of Naval Architecture and Offshore Technology Between Harbin Engineering University, University of Lisbon, Harbin, 150001, China;1. Biochemistry & Molecular Biology Program, Departments of Biology and Chemistry, The College of Wooster, Wooster, OH, United States;2. Department of Microbiology & Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX, United States;1. Department of Neuroradiology, ASST settelaghi, Ospedale di Circolo e Fondazione Macchi, Varese, Italy;2. Department of Radiology, Ospedale di Circolo e Fondazione Macchi, University of Insubria, Varese, Italy;3. Department of Neurosurgery, Ospedale di Circolo e Fondazione Macchi, University of Insubria, Varese, Italy |
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| Abstract: | Tissues from Manduca sexta were examined for the presence of l-ascorbic acid and l-gulonolactone oxidase. l-Ascorbic acid was found in eggs, larval labial gland, haemolymph, gut, muscle, cuticle, adult nervous tissue and gonads at concentrations ranging from < 10 to > 150 mg per 100 g wet tissue. No ascorbate was detected in larval fat body and Malphigian tubule or adult salivary gland. Concentrations in labial gland and haemolymph increased 80- and 10-fold, respectively, during the fifth larval instar such that the labial gland surpassed all other tissues in ascorbate concentration. Since tissues from insects reared on an l-ascorbate-deficient diet contained no detectable vitamin C and l-gulonolactone oxidase was absent from tissue extracts, the hornworm apparently acquired l-ascorbate solely from the diet. |
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