Arachidonic acid in astrocytes blocks Ca(2+) oscillations by inhibiting store-operated Ca(2+) entry,and causes delayed Ca(2+) influx |
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Authors: | Sergeeva Marina Strokin Mikhail Wang Hong Ubl Joachim J Reiser Georg |
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Institution: | Institut für Neurobiochemie, Medizinische Fakult?t der Otto-von-Guericke-Universit?t Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany. |
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Abstract: | ATP-elicited oscillations of the concentration of free intracellular Ca(2+) (Ca(2+)](i)) in rat brain astrocytes were abolished by simultaneous arachidonic acid (AA) addition, whereas the tetraenoic analogue 5,8,11,14-eicosatetraynoic acid (ETYA) was ineffective. Inhibition of oscillations is due to suppression by AA of intracellular Ca(2+) store refilling. Short-term application of AA, but not ETYA, blocked Ca(2+) influx, which was evoked by depletion of stores with cyclopiazonic acid (CPA) or thapsigargin (Tg). Addition of AA after ATP blocked ongoing Ca(2+)](i) oscillations. Prolonged AA application without or with agonist could evoke a delayed Ca(2+)](i) increase. This AA-induced Ca(2+)](i) rise developed slowly, reached a plateau after 5 min, could be reversed by addition of bovine serum albumin (BSA), that scavenges AA, and was blocked by 1 microM Gd(3+), indicative for the influx of extracellular Ca(2+). Specificity for AA as active agent was demonstrated by ineffectiveness of C16:0, C18:0, C20:0, C18:2, and ETYA. Moreover, the action of AA was not affected by inhibitors of oxidative metabolism of AA (ibuprofen, MK886, SKF525A). Thus, AA exerted a dual effect on astrocytic Ca(2+)](i), firstly, a rapid reduction of capacitative Ca(2+) entry thereby suppressing Ca(2+)](i) oscillations, and secondly inducing a delayed activation of Ca(2+) entry, also sensitive to low Gd(3+) concentration. |
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