Influence of photoperiod and medium formulation on peanut somatic embryogenesis |
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Authors: | Charleen M Baker J Austin Burns Hazel Y Wetzstein |
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Institution: | (1) Horticulture Department, University of Georgia, 1111 Plant Science Building, 30602-7273 Athens, GA, USA |
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Abstract: | Somatic embryos were produced from peanut (Arachis hypogaea L.) immature zygotic cotyledons. Comparisons were made of the level of -naphthaleneacetic acid during induction, nitrogen formulation of the medium, and photoperiod. Over 70% embryogenesis was obtained regardless of NAA level used. Percent embryogenesis and number of embryos were markedly lower in explants induced on NAA compared to 2,4-D. Embryo production was not greatly affected by either the use of Murashige & Skoog versus Finer & Nagasawa salts or light versus dark culture conditions. However, embryo morphology was noticeably affected by photoperiod. Embryos produced under a 16 h photoperiod were tough, woody and difficult to separate for subsequent germination and conversion. Those produced under a 0-h photoperiod were succulent and pliable.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
-naphthaleneacetic acid
- MS
Murashige & Skoog (1962)
- B5
Gamborg et al. (1968)
- picloram
4-amino-3,5,6-trichloropicolinic acid
- FN
Finer & Nagasawa (1988)
- 2,4,5-T
2,4,5-trichlorophenoxyacetic acid |
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