FAK and PYK2 interact with SAP90/PSD-95-Associated Protein-3 |
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Authors: | Bongiorno-Borbone Lucilla Kadaré Gress Benfenati Fabio Girault Jean-Antoine |
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Institution: | INSERM, U536, Paris, France. |
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Abstract: | Focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2) are two related non-receptor tyrosine kinases highly expressed in brain. Although they are both involved in synaptic plasticity, little is known about their specific neuronal partners. Using a yeast two-hybrid screen and GST pull-down assays we show that SAPAP3 (SAP90/PSD-95-Associated Protein-3) interacts with FAK (residues 676-840) and PYK2. The three proteins partly co-distribute in the same sucrose gradient fractions as the post-synaptic density protein PSD-95 and Src. Our results suggest that SAPAP3 is an anchoring protein for FAK and PYK2 in post-synaptic densities and may contribute to the synaptic function of these tyrosine kinases. |
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Keywords: | Non-receptor tyrosine kinase Synapses Protein-protein interaction Post-synaptic densities Neurons Src GKAP |
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