Over-expression of strawberry d-galacturonic acid reductase in potato leads to accumulation of vitamin C with enhanced abiotic stress tolerance |
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Authors: | Hemavathi Chandrama Prakash Upadhyaya Ko Eun Young Nookaraju Akula Hyun soon Kim Jeon Jae Heung Oh Man Oh Chenna Reddy Aswath Se Chul Chun Doo Hwan Kim Se Won Park |
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Affiliation: | aDepartment of Molecular Biotechnology, Konkuk University, Seoul 143-701, South Korea;bKorean Research Institute of Bioscience and Biotechnology, Taejeon, South Korea;cDepartment of Plant Biology, USDA-ARS, University of Illinois at Urbana-Champaign, Urbana, USA;dIndian Institute of Horticultural Research, Bangalore, India |
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Abstract: | Vitamin C (ascorbic acid) is an essential component for collagen biosynthesis and also for the proper functioning of the cardiovascular system in humans. Unlike most of the animals, humans lack the ability to synthesize ascorbic acid on their own due to a mutation in the gene encoding the last enzyme of ascorbate biosynthesis. As a result, vitamin C must be obtained from dietary sources like plants. In this study, we have developed transgenic potato plants (Solanum tuberosum L. cv. Taedong Valley) over-expressing strawberry GalUR gene under the control of CaMV 35S promoter with increased ascorbic acid levels. Integration of the GalUR gene in the plant genome was confirmed by PCR and Southern blotting. Ascorbic acid (AsA) levels in transgenic tubers were determined by high-performance liquid chromatography (HPLC). The over-expression of GalUR resulted in 1.6–2-fold increase in AsA in transgenic potato and the levels of AsA were positively correlated with increased GalUR activity. The transgenic lines with enhanced vitamin C content showed enhanced tolerance to abiotic stresses induced by methyl viologen (MV), NaCl or mannitol as compared to untransformed control plants. The leaf disc senescence assay showed better tolerance in transgenic lines by retaining higher chlorophyll as compared to the untransformed control plants. Present study demonstrated that the over-expression of GalUR gene enhanced the level of AsA in potato tubers and these transgenics performed better under different abiotic stresses as compared to untransformed control. |
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Keywords: | Vitamin C Solanum tuberosum L. smCaps" >d-Galacturonic acid reductase HPLC Abiotic stress |
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