实验性小鼠皮肤着色真菌病MCP-1和MIP-2研究

目的观察小鼠皮肤着色真菌病模型发病过程中趋化性细胞因子MCP-1、MIP-2表达的动态变化,探讨其在宿主防御机制中的可能作用。方法 ICR小鼠分为3组,A组为健康小鼠足垫皮下接种灭活卡氏支孢霉悬液(1×108cfu/mL,0.025mL);B组为健康小鼠足垫皮下接种卡氏支孢霉悬液(1×108cfu/mL,0.025mL);C组为免疫抑制小鼠足垫皮下接种卡氏支孢霉悬液(1×108cfu/mL,0.025mL)。接种后第7天、30天、60天时用荧光实时定量PCR法检测皮损组织MCP-1及MIP-2的mRNA表达水平、ELISA法检测皮损组织匀浆中MCP-1及MIP-2蛋白水平。结果接种后卡氏支孢霉悬液的B组7d时MCP-1、MIP-2表达水平明显高于30d、60d,且7d时B组MCP-1、MIP-2表达水平明显高于A组和C组,但30d、60d时A、B、C组之间无显著性差异。结论在卡氏支孢霉所致的着色真菌病模型的发病过程中可能有MCP-1、MIP-2这两种趋化性细胞因子的参与。

Experimental study on the gene expression of MCP-1 and MIP-2 in chromomycosis in mice

Objective To detect the gene expression of chemokines in mice model of skin chromomycosis and explore their roles in protective mechanism of the host. Methods Active or inactive C. carrion suspension （1 × 108 cfu/mL,0. 025mL） was inoculated subcutaneously in the feet pads of healthy or immunosuppressive mice,respectively. mRNAs of MCP-1 and MIP-2 in the leision were examined by a real-time fluorescent quantitative PCR （FQ-PCR）,and protein levels of MCP-1 and MIP-2 in the lesion tissue were examined by ELISA on day 7,30 and 60 after inoculation. Results Expression of MCP-1 and MIP-2 in healthy mice inoculated with active C. carrion on day 7 was higher than that on other days or that in other groups on day 7. But there were no significant differences on day 30 or day 60. Conclusions Both MCP-1 and MIP-2 possiblely participated in the pathogenesis of chromomycosis model due to C. carrion .