Phenobarbital induction of egasyn: availability of egasyn in vivo determines glucuronidase binding to membrane. |
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Authors: | D Owerbach A J Luis |
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Affiliation: | Department of Molecular Biology Roswell Park Memorial Institute Buffalo, New York 14263 USA |
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Abstract: | Murine egasyn, a protein which stabilizes the binding of β-glucuronidase to microsomal membranes, was induced 1.9 fold in liver by phenobarbital treatment. Accompanying this increase was an alteration of the subcellular distribution of liver β-glucuronidase, although total glucuronidase activity remained constant. In control mice 32.6 ± 4.6% of the activity was microsomal, while after four days of phenobarbital treatment 50.5 ± 3.1% was microsomal. Thus, the availability of egasyn appears to be an important factor in determining the proportion of glucuronidase distributed to either microsomes or lysosomes. |
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