Psoralen plus near ultraviolet light inactivation of cultured Chinese hamster cells and its relation to DNA cross-links |
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| Authors: | E Ben-Hur M M Elkind |
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| Affiliation: | 1. Biology Department Brookhaven National Laboratory, Upton, N.Y. 11973 U.S.A.;2. Experimental Radiopathology Unit, Medical Research Council, Hammersmith Hospital, London W12 Great Britain |
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| Abstract: | The survival curve of Chinese hamster cells exposed to near ultraviolet (NUV) light in the presence of 4,5′,8-trimethylpsoralen exhibits a pronounced shoulder, i.e. cells accumulate sublethal damage before survival decreases exponentially. During incubation between fractionated exposures, the cells are able to recover their capacity to accumulate sublethal damage, although at a slower rate than after X-irradiation. Synchronized cells demonstrate a pronounced age-response variation, similar to that of X-irradiated cells, in which maximum resistance occurs in the second half of the S phase. During a second NUV exposure without psoralen, following a first treatment consisting of psoralen-plus-NUV, only DNA cross-links are produced. Using this procedure a correlation was found between cell killing and the production of DNA cross-links. |
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| Keywords: | BUdR 5-bromodeoxyuridine HU hydroxyurea NUV near ultraviolet PE plating efficiency PY percent yield |
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