Microspore cultures as donor tissue for the initiation of embryogenic cell suspensions in barley |
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Authors: | Renate Lührs Kirsten Nielsen |
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Affiliation: | (1) Plant Biology Section, Environmental Science and Technology Department, Risø National Laboratory, DK-4000 Roskilde, Denmark;(2) Present address: Institut für Allgemeine Botanik, Arbeitsbereich Genetik, Ohnhorststrasse 18, D-2000 Hamburg 52, Germany;(3) Present address: Danish Plant Breeding Ltd, Boelshøj, Højerupvej 31, 4660 St. Heddinge, Denmark |
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Abstract: | We have initiated embryogenic cell suspension cultures of barley (Hordeum vulgare L.) Igri from isolated microspore cultures. Data were obtained on the time required for establishment, frequency of establishment, i.e. number of calluses out of the total number of initiations giving rise to suspensions, and embryogenic capacity of the suspension cultures. For comparison, establishment of embryogenic cell suspensions from callus derived from immature zygotic embryos of Igri, Dissa and Golden Promise was also carried out. The results revealed that embryogenic suspension cultures were established in half the time and with a seven-fold higher frequency from microspore cultures than from zygotic embryo-derived calluses. The suspension cultures were still capable of embryo formation after two years. However, only albino plantlets were regenerated. For comparison, long term callus cultures derived from microspores, anthers and zygotic embryos were established. From the anther and zygotic embryo-derived callus cultures green plants were continuously regenerated, whereas the microspore-derived callus cultures lost this ability after the second subculture. |
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Keywords: | Hordeum vulgare plant regeneration suspension culture |
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