| Abstract: | Two fast and efficient purification methods for the preparation of large amounts of proline-specific endopeptidase (PSE) [EC 3.4.21.26] fromFlavobacterium meningosepticum heterologously expressed inEscherichia coli are described. Overproduction and accumulation of PSE in the periplasmic space ofE. coli means that a single gel chromatography step or ion exchange chromatography step was sufficient to obtain homogenously pure PSE preparations. With these procedures, up to 490  g of purified enzyme per gE. coli cells were obtained. The different purification methods are discussed.The authors are with the Weissheimer Research Laboratory, Department of Biotechnology, Schaarstrasse 1, D-56626 Andernach, Germany |
