Naegleria lovaniensis new species: Isolation and identification of six thermophilic strains of a new species found in association with Naegleria fowleri |
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Authors: | A.R. Stevens J. De Jonckheere E. Willaert |
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Affiliation: | 1. Research Service, Veterans Administration Medical Center, Gainesville, Fl 32602, U.S.A.;2. Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, Fl 32610, U.S.A.;3. Katholieke Universteit Leuven, Laboratorium voor Hygiene, Leuven, Belgium |
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Abstract: | Six Naegleria strains, isolated previously in association with N. fowleri from thermal waters, were studied to further determine their antigenic relationship to N. fowleri and other Naegleria species. Results of immunofluorescent antibody and immunoelectrophoretic studies clearly established the antigenic divergence of the variants from N. fowleri, N. gruberi, and N. jadini. The variants were further distinguished from known Naegleria species by several ultrastructural characteristics, which included the complete enclosure of their nuclei with one or several layers of rough endoplasmic reticulum (RER) and extrusion of their nuclear material. Extruded nuclear material was observed in the cytoplasm completely sequestered by cisternae of the RER. The variants were also shown to be sensitive to agglutination induced by concanavalin A but not by wheat germ agglutinin. Based on the differences in the antigenicity, morphology, and lectin sensitivity between these six variants and established Naegleria species, we proposed that they should be established as a new species. |
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Keywords: | nonpathogenic variants cytology indirect immuno-fluorescent antibody technique immunoelectrophoresis ultrastructure nuclear extrusion lectin agglutination concanavalin A wheat germ agglutination new species Address for correspondence or reprints: A.R. Stevens, Ph.D., Research Service (151), Veterans Administration Medical Center, Gainesville, FL 32602, U.S.A.. |
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