Regulatory properties of a partially purified preparation of pyruvate kinase from Fasciola hepatica |
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Authors: | Carolyn A. Behm C. Bryant |
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Affiliation: | Department of Zoology, Australian National University, Box 4 PO, Canberra, ACT 2600, Australia |
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Abstract: | It possesses sigmoid kinetics with PEP; FBP activation changes the relationship to a rectangular hyperbola. The enzyme is inhibited by malate, which competes with PEP; FBP relieves the inhibition slightly. ATP and bicarbonate ions are also inhibitory at high concentrations. ATP inhibition is mixed-competitive with PEP; bicarbonate inhibition is non-competitive. It is suggested that pyruvate kinase may regulate both lactate and acetate production by moderating the size of the cytosolic pyruvate pool. |
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Keywords: | pyruvate kinase metabolic regulation |
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