Cell swelling activates STAT1 and STAT3 proteins in cultured rat hepatocytes |
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Authors: | Meisse D Dusanter-Fourt I Husson A Lavoinne A |
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Institution: | State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Academia Sinica, PR China. |
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Abstract: | According to the known primary sequences of three neurotoxins active on small conductance Ca2+-activated potassium channels from the scorpion Buthus martensi Karsch, their corresponding cDNAs were cloned and sequenced using 3'- and 5'-RACE. All of them encoded a signal peptide composed of 28 residues and a mature toxin of 29, 28 and 33 residues, respectively. Their cDNA deduced sequences were totally consistent with those determined, and the C-terminal amidation of one neurotoxin was confirmed. The genomic DNAs of these three toxins were also amplified by PCR, cloned and sequenced. They all consisted of two exons disrupted by a small single intron. All of these introns were inserted within the signal peptide at the same -10 position upstream from the mature toxin, consisting of 94, 78 and 87 bp, respectively. |
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