Outcome of adrenal tissue fragments allotransplantation: The impact of cryopreservation |
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| Institution: | 1. Institute of Endocrinology, Beilinson Hospital, Petach Tikva, Israel;2. Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel;3. Division of Endocrinology, University Health Network and University of Toronto, Toronto, Ontario, Canada;4. Division of Medical Oncology & Hematology, Princess Margaret Cancer Centre, Toronto, Canada;5. Department of Medicine, University of Toronto, Toronto, Canada;6. Allogeneic Blood and Marrow Transplant Program, Department of Medical Oncology and Hematology, Princess Margaret Cancer Centre, Toronto, Ontario, Canada;7. Department of Endocrine Oncology, Princess Margaret Cancer Centre, Toronto, Ontario, Canada.;1. Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, Toronto, ON, Canada;2. Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada;3. Child & Family Research Institute, Vancouver, BC, Canada;4. Division of Translational Therapeutics, Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada;5. University Institute of Clinical Chemistry, Inselspital Bern University Hospital, University of Bern, Switzerland;6. Winnipeg Regional Health Authority, Pharmacy Department, Winnipeg, Manitoba, Canada;7. Pharmaceutical Outcomes Programme, BC Children''s Hospital, Vancouver, BC, Canada;8. Maccabi-Kahn Research Institute, Tel Aviv, Israel |
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| Abstract: | Cryopreservation is thought to have the potential to preserve tissue for transplantation. In addition, it can also be used for decreasing tissue immunogenicity, which might be important for prolonging allograft survival. In the present study we examined the impact of cryopreservation at various cooling rates on the outcome of allotransplantation of murine adrenal tissue fragments (ATFr). ATFr were cryopreserved with a cooling rate at 1; 10; 40 and more than 100 °C/min. After thawing it was found that the number of the cells expressing markers of dendritic cells (CD11c) and macrophages (CD11b) in the suspension obtained from ATFr decreased with increasing cooling rate. After allotransplantation the survival rates of adrenalectomized mice and the blood serum levels of corticosterone were higher in recipients of cryopreserved ATFr. By immunohistochemistry, cryopreserved allografts displayed a decreased infiltration by CD4+ and CD8+ T-lymphocytes as compared to fresh grafts. These findings suggest that cryopreserved allografts cause a less severe rejection by decreasing graft immunogenicity. |
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