Cryoprotective and contraceptive properties of egg yolk as an additive in rooster sperm diluents |
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| Affiliation: | 1. Dpto. Reproducción Animal, INIA, 28040 Madrid, Spain;2. Dpto. Mejora Genética Animal, INIA, 28040 Madrid, Spain;1. Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand;2. Research and Development Network Center for Animal Breeding (Native Chicken), Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand;1. Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran;2. Department of Poultry Science, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran;3. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;4. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;1. Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran;2. Department of Poultry Science, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran;3. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;4. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;5. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;1. Department of Poultry Production, Faculty of Agriculture, Assiut University, 71526 Assiut, Egypt;2. Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria (INIA), 28040 Madrid, Spain |
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| Abstract: | The addition of chicken egg yolk to semen extenders is thought to reduce the fertilizing potential of rooster spermatozoa - but not (or at least not as much) that of other avian species. The aim of the present study was to determine whether quail egg yolk, a novel extender additive, provides advantages over chicken egg yolk in the cryopreservation of rooster spermatozoa. Experiments were also performed to determine whether the harmful effect of egg yolk occurs during cryopreservation or during fertilization after artificial insemination. Heterospermic rooster semen samples were divided into aliquots and cooled in a polyvinylpyrrolidone-based medium containing 15% chicken egg yolk, 15% quail egg yolk or no egg yolk at all. The viability of spermatozoa of cooled samples (5 °C) without egg yolk were less viable (P < 0.01) than those of samples containing either type of egg yolk. The same aliquots were then cryopreserved for 15 days. Thawed spermatozoa preserved without egg yolk showed lower motility (P < 0.001) and viability (P < 0.001) than those in samples diluted with either type of egg yolk extender. No eggs were fertilized when hens were inseminated with semen that had been diluted with chicken egg yolk. The fertilization rate was only slightly higher when sperm diluted with quail egg yolk was used (1.5%). The best results were obtained when no egg yolk was used (13.8%). These results show that the addition of egg yolk of either type protects rooster sperm cells against cold shock and during freezing and thawing, but exerts a contraceptive effect in the genital tract of the hen. |
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