Monoclonal antibodies specific for cell culture mycoplasmas |
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Authors: | David W Buck Roger H Kennett Gerard McGarrity |
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Institution: | (1) Cetus Corporation, 600 Bancroft Way, 94710 Berkeley, CA;(2) Cell Center of University of Pennsylvania Human Genetics Center, and Institute for Medical Research, Camden, New Jersey |
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Abstract: | Summary Mycoplasma infection of cell cultures is still a major problem in some laboratories. Although several methods can be used
for their detection, identification is normally by serological procedures. As no commercial source for the necessary antibodies
is available we have prepared monoclonal antibodies to the five mycoplasma species that account for the majority of cell culture
infections. These antibodies have been characterized by the growth inhibition test (GIT), immunofluorescence, and enzyme linked
immunosorbent assay (ELISA) and have shown perfect correlation in all tests when compared to conventional antisera raised
in rabbits or donkeys. In addition, a monoclonal antibody toMycoplasma pneumoniae was produced.M. pneumoniae is an infrequent cell culture contaminant but is a human pathogen, and the monoclonal antibody described here could be useful
in the clinical diagnosis ofM. pneumoniae infection in man.
These studies were supported by Grants Al-15748 from the National Institute of Allergy and Infectious Diseases, and GM20138-07
from the National Institutes of Health, Bethesda, MD. |
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Keywords: | mycoplasma monoclonal antibodies detection speciation |
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