Vero 细胞流感病毒的大规模培养和纯化

目的：采用反应器技术以细胞为基质培养流感病毒，并探索其纯化工艺。 方法：采用5L反应器无血清条件下以Vero细胞为基质培养流感病毒，病毒收获后，经灭活、澄清，采用阴离子柱去除宿主DNA，亲和柱浓缩流感病毒，最后采用分子筛三步层析法进行纯化。结果：整个纯化工艺HA回收率达102%，蛋白降低率为95.3%，宿主蛋白降低率为99.77%，DNA的降低率为99.99%。结论 本研究成功建立了一种细胞流感病毒的纯化工艺。

Mass Culture and Purification of Vero-based Influenza Virus

Objective Establish mass-scale purification technology of cell-derived influenza virus. Methods A microcarrier-based process was used to produce human influenza virus in serum free-adapted Vero cells. The virus was purified in a sequence of downstream processing steps including inactivation, clarification, anion exchange chromatography, affinity chromatography and size-exclusion chromatography. Results The recovery of HA reached 102%. 95.3% total protein, including 99.77% host cell protein, and 99.99% host cell DNA were removed during downstream processing. Conclusion This research provides a high effective purification technology for cell-derived influenza virus.