Effects of resveratrol on biomarkers of oxidative stress and on the activity of delta aminolevulinic acid dehydratase in liver and kidney of streptozotocin-induced diabetic rats |
| |
Authors: | Schmatz Roberta Perreira Luciane Belmonte Stefanello Naiara Mazzanti Cinthia Spanevello Roselia Gutierres Jessié Bagatini Margarete Martins Caroline Curry Abdalla Fátima Husein Daci da Silva Serres Jonas Zanini Daniela Vieira Juliano Marchi Cardoso Andréia Machado Schetinger Maria Rosa Morsch Vera Maria |
| |
Affiliation: | 1. Programa de Pós Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil;2. Colegiado do curso de Enfermagem, Universidade Federal da Fronteira Sul, Campus Chapecó, Chapecó, SC, Brazil;3. Universidade Federal de Pelotas, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Setor de Bioquímica, Campus Universitário Capão do Leão 96010-900 Pelotas, RS, Brazil |
| |
Abstract: | The present study investigated the effects of resveratrol (RV), a polyphenol with potent antioxidant properties, on oxidative stress parameters in liver and kidney, as well as on serum biochemical parameters of streptozotocin (STZ)-induced diabetic rats. Animals were divided into six groups (n = 8): control/saline; control/RV 10 mg/kg; control/RV 20 mg/kg; diabetic/saline; diabetic/RV10 mg/kg; diabetic/RV 20 mg/kg. After 30 days of treatment with resveratrol the animals were sacrificed and the liver, kidney and serum were used for experimental determinations. Results showed that TBARS levels were significantly increased in the diabetic/saline group and the administration of resveratrol prevented this increase in the diabetic/RV10 and diabetic/RV20 groups (P < 0.05). The activities of catalase (CAT), superoxide dismutase (SOD) and aminolevulinic acid dehydratase (δ-ALA-D) and the levels of non protein thiols (NPSH) and vitamin C presented a significant decrease in the diabetic/saline group when compared with the control/saline group (P < 0.05). The treatment with resveratrol was able to prevent these decrease improving the antioxidant defense of the diabetic/RV10 and diabetic/RV20 groups (P < 0.05). In addition, the elevation in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamiltransferase (γ-GT) activities as well as in levels of urea, creatinine, cholesterol and triglycerides observed in the diabetic/saline group were reverted to levels close to normal by the administration of resveratrol in the diabetic/RV10 and diabetic/RV20 groups (P < 0.05). These findings suggest that resveratrol could have a protector effect against hepatic and renal damage induced by oxidative stress in the diabetic state, which was evidenced by the capacity of this polyphenol to modulate the antioxidant defense and to decrease the lipid peroxidation in these tissues. |
| |
Keywords: | Diabetes Resveratrol Oxidative stress Aminolevulinic acid dehydratase Liver |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|