Similar degree of methyl methanesulphonate-induced DNA damage in two clones ofTradescantia differing in mutagenic sensitivity to alkylating agents |
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Authors: | J Velemínský T Gichner V Pokorný J Šatava |
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Institution: | (1) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Flemingovo n. 2, 160 00 Praha 6, Czechoslovakia |
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Abstract: | In two clones ofTradescantia (4430 and 02) differing in the sensitivity to the mutagenic action of alkylating agents, equimolar doses of 14C] methyl methanesulphonate (MMS) elicited a similar degree of protein, RNA and DNA alkylation and a similar amount of DNA-7-methylguanine
and DNA-3-methyladenine in cells of inflorescence. Moreover, in the same clones and tissues the same doses of nonlabelled
MMS produced a similar amount of DNA single strand breaks and/or alkali labile sites as measured in alkaline sucrose gradients.
None of the DNA lesions followed is therefore decisive for explanation of the different mutagenic sensitivity ofTradescantia clones. |
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