| 红外光谱法在基因探测中的应用研究 |
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| 引用本文： | 王黎明,赵芬芝,胡永忠. 红外光谱法在基因探测中的应用研究[J]. 中国生物化学与分子生物学报, 1986, 2(5): 49-55 |
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| 作者姓名： | 王黎明 赵芬芝 胡永忠 |
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| 作者单位： | 天津市计划生育研究所(王黎明)，南开大学高分子化学研究所(赵芬芝)，南开大学高分子化学研究所(胡永忠) |
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| 摘 要： | 应用干涉型傅里叶变换红外分光光度计及聚乙烯膜作载体,得到了单链、双链DNA、生物素-dUTP及经过缺口翻译参入生物素的DNA探针的红外光谱,观察到Bio-dUTP分子内化学键振动产生的特征吸收谱带。利用差谱技术比较了DNA与生物素标记DNA的差异。Bio-dUTP的特征吸收探测灵敏度可达0.1ng。同时分析了硝酸纤维膜与genescreen的红外透过性质。
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| 关 键 词： | 红外光谱 生物素标记DNA 分子内特征振动 特征吸收 差谱技术 |
| 收稿时间： | 1986-10-20 |
RESEARCH ON APPLICATION OF INFRARED TECHNIQUE IN GENE ANALYSIS |
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| Wang,Li-ming. RESEARCH ON APPLICATION OF INFRARED TECHNIQUE IN GENE ANALYSIS[J]. Chinese Journal of Biochemistry and Molecular Biology, 1986, 2(5): 49-55 |
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| Authors: | Wang Li-ming |
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| Affiliation: | (Institute of Family Planning,Tianjin)Zhao,Fen-zhi Hu,Yong-zhong(Institute of Polymer Chemistry,Nankai University,Tianji |
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| Abstract: | By means of improved free-standing film technique and Fourier Transform Infrared spectrometer,infrared spectra of single strand,double strand and biotin labeled DNAs were investigated; characteristic absorbances generated by specific chemical bond vibrations within biotin molecule were observed with sensitivity of 0.1ng Bio-dUTP,which might be used in DNA analysis or pathogen detection by Southern or dot blot hybridzation.The advantages and possibilities of applying FTIR technique in molecular biology were discussed. |
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| Keywords: | Infrared spectrum Biotin labeled DNA Inter-molecular vibration Characteristic absorbance Spectral subtraction. |
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