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基因组后研究策略
引用本文:杜光伟,袁建刚,强伯勤.基因组后研究策略[J].生物化学与生物物理进展,1997,24(2):100-103.
作者姓名:杜光伟  袁建刚  强伯勤
作者单位:中国医学科学院中国协和医科大学基础医学研究所,医学分子生物学国家重点实验室,北京 100005;中国医学科学院中国协和医科大学基础医学研究所,医学分子生物学国家重点实验室,北京 100005;中国医学科学院中国协和医科大学基础医学研究所,医学分子生物学国家重点实验室,北京 100005
基金项目:国家自然科学基金重大项目资助.
摘    要:由于基因组计划的进展,数据库中积累了越来越多不知道功能的基因序列,分析这些基因的功能将成为基因组计划完成后的主要任务.文章介绍了几种大规模分析基因功能的策略及程序:基因插断、基因表达的系统分析、高密度cDNA杂交、蛋白组分析,并讨论了大规模基因功能分析的前景.

关 键 词:基因组后,基因的功能分析,基因插断,基因表达的系统分析,高密度cDNA杂交,蛋白组分析
收稿时间:4/2/1996 12:00:00 AM
修稿时间:1996/7/25 0:00:00

Strategies for Postgenome Reasearch
DU Guangwei,YUAN Jiangang and QIANG Boqin.Strategies for Postgenome Reasearch[J].Progress In Biochemistry and Biophysics,1997,24(2):100-103.
Authors:DU Guangwei  YUAN Jiangang and QIANG Boqin
Institution:National Laboratry of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China;National Laboratry of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China;National Laboratry of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China
Abstract:Attributing to the progress in the Genome Project, more and more gene sequences, most of which are not known any information about function, have been accumulated in database. So analysing the function of those genes will become the main goal after the Genome Project is finished. Some strategies and procedures for large-scale functional analyses of genes, such as gene disruptions, serial analysis of gene expression,high density cDNA hybridization and proteome analyses, are introduced. Furthermore, the prospects of large-scale gene functional analyses are also discussed.
Keywords:postgenome  functional analyses of genes  gene disruptions  SAGE  high density cDNA hybridization  proteome analyses
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