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Biochemical and Histochemical Studies on Catecholamines in Bullfrog Spinal Ganglia
Authors:H Kojima  S Fukushima  T Hirono  K Suetake  N Toshima  M Yoshida  S Anraku  K Inanaga
Institution:Institute of Brain Diseases, Kurume University School of Medicine, Kurume, Japan;Department of Sanitary Technology, Ginkyo College of Medical Technology, Kumamoto, Japan
Abstract:Abstract: Bullfrog spinal ganglia were subjected to two types of examination to determine whether catecholamines were present. A biochemical microassay developed by Kissinger and co-workers and a histofluorescence technique for cellular demonstration of biogenic monoamines developed earlier by Falck and co-workers were used. Bullfrog spinal ganglia and dorsal roots were found to contain catecholamines, primarily adrenaline.
Keywords:Bullfrog spinal ganglia  Catecholamine  High performance liquid chromatography  Fluorescence histochemical analysis
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