Immunocytochemical and chemical analyses of Golgi vesicles isolated from the germinated pollen ofCamellia japonica |
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Authors: | Yoshikazu Hasegawa Sumio Nakamura Sayuri Kakizoe Masayuki Sato Norio Nakamura |
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Institution: | (1) Graduate School of Integrated Science, Yokohama City University, 236 Yokohama, Japan;(2) Biological Laboratory, Kanagawa Dental College, 238 Yokosuka, Japan;(3) Department of Biology, Yokohama City, University, Kanazawa-ku, 236 Yokohama, Japan |
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Abstract: | As a first step towards studying the biochemical relationship between Golgi vesicles (GVs) and tube wall components, isolation
of GVs from the growing pollen tubes ofCamellia japonica was attempted using a centrifugation method with mannitol. The isolated GV was identified ultrastructurally and immunocytochemically.
The main components of the GV were proteins and carbohydrates. The main monosaccharides of GV polysaccharides were galactose,
arabinose and uronic acid, and pectins and arabinogalactan proteins also were detected immunochemically. An antiserum against
the isolated GVs reacted with the outer layer of the pollen tube wall and the intine layers of the grain wall as well as thein situ GVs in the pollen tube and the grain cytoplasm. We have thus successfully isolated GVs and shown that they contain pectic
substances and arabinogalactan proteins which contribute to formation of the pollen tube primary wall. |
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Keywords: | Camellia japonica Golgi vesicle component Immunocytochemistry Pollen tube wall formation |
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