Genetic and biochemical studies on glutamate-pyruvate transaminase from Drosophila melanogaster |
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Authors: | Andrew J. Leigh Brown Robert A. Voelker |
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Affiliation: | (1) Laboratory of Animal Genetics, National Institute of Environmental Health Sciences, 27709 Research Triangle Park, North Carolina;(2) Present address: Imperial Cancer Research Fund, Mill Hill Laboratories, NW 7 London, United Kingdom |
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Abstract: | We have used electrophoretic variants of glutamate-pyruvate transaminase (GPT, E.C. 2.6.1.2) in Drosophila melanogaster to genetically map the structural gene to position 42.6 on the X chromosome. By pseudodominance tests over several deficiencies we have localized it cytogenetically to the interval 11Fl-2 to 12Al-2. The sedimentation constant (s20,w) of the native enzyme was determined in sucrose density gradients to be 5.9 and the native molecular weight approximately 87,000. The similarity in physical properties to mammalian enzymes suggests that the enzyme may also be dimeric in D. melanogaster. |
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Keywords: | Drosophila melanogaster allozymes GPT genetic mapping |
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