The immunoassay of gibberellins |
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Authors: | Rainer Atzorn Elmar W. Weiler |
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Affiliation: | (1) Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität, Postfach 102148, D-4630 Bochum, Federal Republic of Germany |
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Abstract: | The development of sensitive and specific solid-phase enzyme immunoassays for gibberellic acid and gibberellins A4 and A7 is reported. The use of antisera of high apparent affinity (Ka over 1010 l mol-1) in conjunction with alkaline phosphatase-labeled gibberellins allows, with minimum procedural effort, the quantitative determination of sub-picogram amounts of these gibberellins. The assays reported here are applicable to most gibberellins and can be set up with 1–1.5 mg of starting material. They represent the most sensitive methods for gibberellin determination known.Abbreviations GA gibberellin - GA3 gibberellic acid - TLC thin-layer-chromatography |
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Keywords: | Enzyme immunoassay (gibberellin) Gibberellin (enzyme immunoassay) |
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