Transfer of Ti plasmids between Agrobacterium strains by mobilisation with the conjugative plasmid RP4 |
| |
Authors: | N Van Larebeke Ch Genetello J P Hernalsteens A De Picker I Zaenen E Messens M Van Montagu and J Schell |
| |
Institution: | (1) Laboratorium voor Histologie en Genetica, Rijksuniversiteit Gent, Ledeganckstraat, 35, B-9000 Gent, Belgium;(2) Laboratorium voor Genetica, Rijksuniversiteit Gent, Ledeganckstraat, 35, B-9000 Gent, Belgium;(3) Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, Paardestraat, 65, B-1640 St.-Genesius Rode, Belgium |
| |
Abstract: | Summary The P type conjugative plasmid RP4 has been shown to be able to promote the transfer of the Agrobacterium Ti-plasmid. The results provide additional evidence that agrocin 84 sensitivity, exclusion of phage AP1, ability to catabolize the guanidine derivatives octopine and nopaline and tumor inducing ability, are Ti-plasmid determined properties. Furthermore, the results strongly support the notion that at least part of the Ti-plasmid is transferred from the bacterium to the target plant cells, since it was demonstrated that Ti-plasmid linked genes specify the synthesis of octopine or nopaline by crown-gall tumor cells. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|