Strange kinetic phase in the extremely early folding process of beta-lactoglobulin |
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Authors: | Kamatari Yuji O Nakamura Hironori K Kuwata Kazuo |
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Institution: | Division of Prion Research, Center for Emerging Infectious Diseases, Gifu University, Gifu 501-1194, Japan. |
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Abstract: | A continuous-wave probed laser-induced temperature jump system was constructed and applied to monitor the changes in tryptophan fluorescence of the beta-lactoglobulin during its folding; the kinetic phases were traced from 300 ns to 10 ms after a temperature jump. Notably, an early phase with typical squeezed-exponential characteristics, exp-(kt)(beta)], beta>1.0], was observed around several tens of microseconds after the temperature jump, which is actually the earliest phase ever observed for beta-lactoglobulin. This process can be explained by conformational shift occurring within the unfolded ensemble (U-->U'), which is followed by the non-native intermediate (I) formation of this protein. |
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Keywords: | cT-jump continuous-wave probed laser-induced temperature jump CD circular dichroism NATA N-acetyltryptophanamide CF continuous-flow PGK phosphoglycerate kinase |
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