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Strange kinetic phase in the extremely early folding process of beta-lactoglobulin
Authors:Kamatari Yuji O  Nakamura Hironori K  Kuwata Kazuo
Institution:Division of Prion Research, Center for Emerging Infectious Diseases, Gifu University, Gifu 501-1194, Japan.
Abstract:A continuous-wave probed laser-induced temperature jump system was constructed and applied to monitor the changes in tryptophan fluorescence of the beta-lactoglobulin during its folding; the kinetic phases were traced from 300 ns to 10 ms after a temperature jump. Notably, an early phase with typical squeezed-exponential characteristics, exp-(kt)(beta)], beta>1.0], was observed around several tens of microseconds after the temperature jump, which is actually the earliest phase ever observed for beta-lactoglobulin. This process can be explained by conformational shift occurring within the unfolded ensemble (U-->U'), which is followed by the non-native intermediate (I) formation of this protein.
Keywords:cT-jump  continuous-wave probed laser-induced temperature jump  CD  circular dichroism  NATA  N-acetyltryptophanamide  CF  continuous-flow  PGK  phosphoglycerate kinase
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