Total nitrogen determining for plant material containing nitrate |
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Authors: | E F Eastin |
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Affiliation: | Texas Agricultural Experiment Station, Route S, Box 784, Beaumont, Texas 77706 USA |
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Abstract: | A nonfoaming method for semimicro Kieldahl determination of total nitrogen in plant samples containing appreciable amounts of nitrate was developed for use with a digestion block utilizing test tubes for digestion flasks. The sample (30 to 200 mg) is treated with 5 ml of a sulfuric acid:salicylie acid (30:1 ) mixture at room temperature for 1 hr. Catalyst [K2SO4:CuSeO3·2H2O:pumice 970:19:11 w/w/w)] is added and the mixture is digested at 360 to 380°C for 1 hr after the mixture clears. Ammonium in the digest is determined by a suitable method. This semimicro Kjeldahl procedure results in a 95% or better recovery of nitrate, either from KNO3 or from KNO3 added to plant material. |
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