Characterization of a group I intron in the nuclera rDNA differentiatingPhialophora gregata f. sp.adzukicola fromP. gregata f. sp.sojae |
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| Authors: | Weidong Chen Lynn E Gray Craig R Grau |
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| Institution: | (1) Illinois Natural History Survey, University of Illinois at Urbana-Champaign, 607 East Peabody Drive, 61820 Champaign, IL, USA;(2) Department of Crop Sciences, University of Illinois at Urbana-Champaign, 607 East Peabody Drive, 61820 Champaign, IL, USA;(3) USDA-ARS, Department of Crop Sciences, University of Illinois at Urbana-Champaign, 1101 West Peabody Drive, 61801 Urbana, IL, USA;(4) Department of Plant Pathology, University of Wisconsin, 1630 Linden Drive, 53706 Madison, WI, USA |
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| Abstract: | An insertion sequence was detected near the 3′ end of the nuclear small subunit rDNA in isolates ofPhialophora gregata f. sp.adzukicola, the causal agent of the brown stem rot disease of adzuki bean. This insertion sequence was absent in isolates ofP. gregata, f. sp.sojae which causes brown stem rot of soybean. The insertion sequence is 304 bp long and contains all the characteristics of group
I introns. These characteristics include, the four conserved sequence elements (P, Q, R, and S), a U at the 5′ splice site
of the exon, a G at the 3′ splice site of the intron, a putative internal guiding sequences; the sequence also fits a secondary
structure model for group I introns. Similar to most group I introns found in nuclear small subunit rDNA, the intron was located
in a highly conserved region and is devoid of long open reading frames. This intron provides a convenient marker for use in
conventional PCR to separateP. gregata f. sp.adzukicola fromP. gregata f. sp.sojae. |
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| Keywords: | brown stem rot group I intron molecular identification ribosomal DNA |
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