DNA repair test of disinfectants by liquid rec-assay |
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| Authors: | Y Sakagami K Yamasaki H Yokoyama Y Ose T Sato |
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| Institution: | Division of Pharmaceutical Affairs, Osaka Prefectural Institute of Public Health, Japan. |
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| Abstract: | The DNA-damaging capacity and the mutagenicity of 6 disinfectants were studied by liquid rec-assay and Ames test. 5 disinfectants were found to be positive in DNA-damaging capacity while only one of them showed clear mutagenicity in the Ames test. Liquid rec-assay by direct incubation with S9 mix was the most sensitive method and gave the best correlation between the growth ratio (R 50) and the time lag, both of which compared Rec+ and Rec-. Liquid rec-assay may be useful for detecting the DNA-damaging capacity of chemicals with a strong killing effect. |
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