| 摘 要： | Summary The effect of 3′methyl-4-dimethylaminoazobenzene (3′-Me-DAB) in the induction of malignant transformation and of 8-azaguanine-resistant
mutations and chromosomal aberrations was studied in a diploid strain derived from normal rat liver cells. The cells were
malignantly transformed by treatment with 3′-Me-DAB 1.7 μg/ml for 130 to 221 d or 1.7 μg/ml for 53 d followed by 24.9 μg/ml
for 27 to 77 d. The untreated control cells did not transform spontaneously until the 232nd d in culture. Some properties
of the 3′-Me-DAB-treated cells were compared to those of untreated control cells but no reliable marker for predicting the
tumorigenic potential of the cells was found.
The single addition of 3′-Me-DAB caused little induction of 8-azaguanine-resistant mutations and chromosomal aberrations to
the cells. However, mutations and chromosomal aberrations were significantly induced byN-acetoxy-4-methylaminoazobenzene, an active metabolite of 4-dimethylaminoazobenzene or 3′-Me-DAB in the presence of liver
microsomes.
This study was supported by a grant for cancer research from the Japanese Ministry of Education.
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