Synthesis and properties of novel 2'-O-alkoxymethyl-modified nucleic acids |
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Authors: | Arai Koichiro Uchiyama Naoki Wada Takeshi |
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Affiliation: | Department of Medical Genome Sciences, The University of Tokyo, Bioscience Building 702, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan. |
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Abstract: | Novel 2'-O-modified oligoribonucleotides with alkoxymethyl skeletons were synthesized, and their ability to hybridize complementary nucleic acids and their nuclease resistance were analyzed. The hybridization ability was improved by introducing electron-withdrawing groups and the increases in melting temperature (T(m) value) was particularly high for chlorine-substituted compounds. Nuclease resistance of these 2'-O-alkoxymethylated oligomers was lower than expected, but cyano substitution resulted in a higher nuclease resistance than 2'-O-methylation. |
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Keywords: | RNA analogs 2′-O-Modification 2′-O-Alkoxymethyl skeleton Duplex stability |
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