Identification, purification and immunohistochemical detection of the inhibitor from porcine ovarian follicular fluid to compensatory ovarian hypertrophy in mice. |
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Authors: | E Sato H Miyamoto T Ishibashi A Iritani |
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Abstract: | An inhibitor to compensatory ovarian hypertrophy in mice was detected in porcine follicular fluid. Compensatory hypertrophy was inhibited by treatment with 0.6 ml whole follicular fluid, 0.2 ml of the non-dialysable fraction, or a fraction of the fluid salted out by ammonium sulphate at a saturation of 14.5--18.5%. The salted-out fraction was separable into two peaks by Sephadex G-200 column chromatography and the second peak, detectable as a single band by polyacrylamide gel disc electrophoresis, contained all the inhibitory activity. Specific fluorescence of an antiserum to the second peak was demonstrated at the granulosa cells. |
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