Postnatal Development of Neurons,Interneurons and Glial Cells in the Substantia Nigra of Mice |
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Authors: | Manami Abe Hiroki Kimoto Risa Eto Taeko Sasaki Hiroyuki Kato Jiro Kasahara Tsutomu Araki |
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Institution: | (1) Department of Neurobiology and Therapeutics, Graduate School and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78, Sho-machi, Tokushima 770-8505, Japan;(2) Department of Neurology, Organized Center of Clinical Medicine, International University of Health and Welfare Hospital, Tochigi, Japan; |
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Abstract: | We investigated postnatal alterations of neurons, interneurons and glial cells in the mouse substantia nigra using immunohistochemistry.
Tyrosine hydroxylase (TH), neuronal nuclei (NeuN), parvalbumin (PV), neuronal nitric oxide synthase (nNOS), glial fibrillary
acidic protein (GFAP), ionized calcium-binding adaptor molecule 1 (Iba 1), CNPase (2′,3′-cyclic nucleotide 3′-phosphodiesterase),
brain-derived neurotrophic factor (BDNF) and glial cell-line-derived neurotrophic factor (GDNF) immunoreactivity were measured
in 1-, 2-, 4- and 8-week-old mice. In the present study, the maturation of NeuN-immunopositive neurons preceded the production
of TH in the substantia nigra during postnatal development in mice. Furthermore, the maturation of nNOS-immunopositive interneurons
preceded the maturation of PV-immunopositive interneurons in the substantia nigra during postnatal development. Among astrocytes,
microglia and oligodendrocytes, in contrast, the development process of oligodendrocytes is delayed in the substantia nigra.
Our double-labeled immunohistochemical study suggests that the neurotrophic factors such as BDNF and GDNF secreted by GFAP-positive
astrocytes may play some role in maturation of neurons, interneurons and glial cells of the substantia nigra during postnatal
development in mice. Thus, our findings provide valuable information on the development processes of the substantia nigra. |
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