| 羊驼催乳素基因cDNA克隆及序列分析 |
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| 引用本文： | 董常生,王海东,姜俊兵,贺俊平,赫晓燕.羊驼催乳素基因cDNA克隆及序列分析[J].激光生物学报,2006,15(5):506-510. |
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| 作者姓名： | 董常生 王海东 姜俊兵 贺俊平 赫晓燕 |
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| 作者单位： | 山西农业大学动物科技学院,山西,太谷,030801;山西农业大学动物科技学院,山西,太谷,030801;山西农业大学动物科技学院,山西,太谷,030801;山西农业大学动物科技学院,山西,太谷,030801;山西农业大学动物科技学院,山西,太谷,030801 |
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| 基金项目： | 农业部国际先进农业科学技术项目(948项目)，“羊驼的引种、繁育和推广技术(NO.202096)和羊驼胚胎移植技术的引进和应用(NO.2003-Z86)”提供资助 |
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| 摘 要： | 实验通过克隆分析羊驼催乳素基因的部分序列,对羊驼催乳素基因的结构和功能进行初步探索和揭示。从GeneBank中已报到的脊椎动物催乳素基因保守区设计一对引物,采用Trizol法提取羊驼胎盘总RNA,利用RT-PCR技术扩增出长度约为510 bp的片断。测序后在NCB I工作平台中进行BLASTn同源性比较,得出结论:羊驼催乳素基因与已登录的哺乳动物催乳素基因同源性均超过85%,最高达97%。借助DNAstar分子生物学分析软件绘制了相关动物的遗传进化图,并对羊驼的种属地位进行了进一步验证。。
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| 关 键 词： | 中华羊驼 催乳素基因 克隆 序列分析 |
| 文章编号： | 1007-7146(2006)05-0506-05 |
| 收稿时间： | 2006/4/10 |
| 修稿时间： | 2006年4月10日 |
Molecular Cloning and Analysis of Alpaca Prolactin Gene Sequences |
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| DONG Chang-sheng,WANG Hai-dong,JIANG Jun-bing,HE Jun-ping,HE Xiao-yan.Molecular Cloning and Analysis of Alpaca Prolactin Gene Sequences[J].ACTA Laser Biology Sinica,2006,15(5):506-510. |
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| Authors: | DONG Chang-sheng WANG Hai-dong JIANG Jun-bing HE Jun-ping HE Xiao-yan |
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| Abstract: | By cloning and analyzing the partial sequence of alpaca prolactin(PRL),the purpose of this research is to qust for framework and function.According to conservative domain of the published sequence of prolactin gene of vertebrate in GeneBank,a pair of primers that can amplify alpaca PRL gene was designed and synthesized.The tatol RNA was extracted by Trizol.The fragments cDNA of about 510 bp was amplyfied by RT-PCR.The sequence was compared with published PRL genes in GeneBank by Blastn,the sequence showed hightest homology with PRL gene.All homology are over 85 % and the highest is 97 %.In virtue of DNAstar molecular biology analysis software,phylogenetic tree of different species animal PRL gene was protracted. |
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| Keywords: | alpaca prolactin gene cloning sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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