The Effect of Cadmium on Tobacco Cell Culture and the Selection of Potentially Cd-Resistant Cell lines |
| |
Authors: | Eva DomaZlická Z OpatrnÝ |
| |
Institution: | (1) Agriculture University, Department of Botany and Plant Physiology, Praha 6, 165 21 Suchdol;(2) Research Institute of Crop Production, Praha 6, Ruzyně, Czechoslovakia |
| |
Abstract: | The effect of different concentrations of cadmium on the viability, cell division, and the total increase in the biomass of
the VBI-O cell strain of tobacco (Nicotiana tabacumL., Virginia Bright Italia) was followed.
The concentration of 10-6 mol 1-1 Cd2+ was fully tolerated by this strain, a nearly total inhibition of cell division and high cell mortality rate ocsicurred at
the concentration of 10-4 mol1{si-1} Cd2+.
Following a long-term exposure of the culture to gradually increasing cadmium concentrations, seven cell lines able to grow
on media containing 10-4 mol 1-1 Cd2+ were derived. Phenotype diversity of the isolated cell lines likely causes of the disappearance of the resistance character
are discussed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|