Cholesterol-loaded cyclodextrin inhibits premature acrosomal reactions in liquid-stored rabbit spermatozoa |
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| Authors: | Serin Ilker Aksoy Melih Ceylan Ahmet |
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| Affiliation: | 1. Department of Neurology, The First Affiliated Hospital and Research Institute of Experimental Neurobiology, Wenzhou Medical College, No. 2 Fuxue Lane, Wenzhou City, Zhejiang Province 325000, PR China;2. The First People''s Hospital of Xiaoshan, No. 199 Shixin South Road, Xiaoshan, Hangzhou, Zhejing Province, 311200, PR China;3. Institute for Biological Sciences, National Research Council of Canada, 1200 Montreal Road, Bldg. M54, Ottawa, Ontario, Canada K1A 0R6;1. Animal & Grassland Research & Innovation Centre, Teagasc, Mellows Campus, Athenry, Co. Galway, Ireland;2. School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland;3. School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland;1. Selcuk University, Faculty of Veterinary Medicine, Department of Parasitology, Konya, Turkey;2. Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Microbiology, Burdur, Turkey;3. Selcuk University, Department of Internal Medicine, Faculty of Veterinary Medicine, Konya, Turkey;1. Unitat de Cunicultura, Institut de Recerca i Tecnologia Agroalimentàries, Torre Marimon s/n, 08140 Caldes de Montbui, Barcelona, Spain;2. Artificial Intelligence Center, University of Oviedo at Gijón, Asturias, Spain;3. Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain |
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| Abstract: | The effect of pretreatment of rabbit sperm cells with different concentrations of cholesterol-loaded cyclodextrin (CLC) on the occurrence of premature acrosome reactions during 72 h of liquid storage was investigated in three successive experiments. The aim of the first experiment was to establish a liquid storage model to facilitate premature acrosome reactions in rabbit sperm cells and, therefore, examined the relative effects of different dilution rates (1:5, 1:25 or 1:50) and storage temperatures (4°C or 35°C) on the occurrence of premature acrosome reactions. Increasing both dilution rate (from 1:5 to 1:25; P<0.05) and storage temperature (from 4°C to 35°C; P<0.0001) significantly enhanced the percentage of sperm cells that underwent premature acrosome reactions during storage. Therefore, a constant dilution rate of 1:25 and storage temperature of 35°C was employed for the rest of the study. The second experiment examined the effect of different CLC concentrations (0, 0.5, 1.0 and 3.0mg per 120×10(6) spermatozoa) on the occurrence of premature acrosome reactions in sperm cells. CLC supplementation of the extender inhibited (P<0.001) premature acrosome reactions in sperm cells in a dose-dependent manner during 72 h of storage. In the third experiment, the ability of CLC-pretreated sperm cells to undergo acrosome reactions induced by lysophosphatidylcholine (LPC) was evaluated following 72 h of storage. A considerable proportion of sperm cells pretreated with CLC (between 68.7 and 91.8%) underwent the acrosome reaction in response to LPC following 72 h of liquid storage. However, the ability of the sperm cells to undergo the acrosome reaction varied with regards to the dose levels of CLC pretreatment (P<0.001). In conclusion, CLC supplementation prevents premature acrosome reactions in liquid-stored rabbit spermatozoa. |
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