Incorporation of a fatty acid containing a photosensitive group into lipids of cultured cardiac cells from chick embryo |
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Authors: | A Franchi G Ailhaud |
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Affiliation: | Centre de Biochimie, Faculté des Sciences, Parc Valrose — 06034 Nice — France |
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Abstract: | Radioactive 12-(4-azido-2-nitrophenoxy)-stearic acid (NAP-stearate) was synthetized; it behaves as a competitive inhibitor of long-chain fatty acids for the entry into cultured cardiac cells from chick embryo. After uptake, [3H] NAP-stearate was incorporated by an energy-dependent process into neutral and polar lipids. Photoactivation as a function of time leads to a covalent labelling of the cells: up to 31 per cent of the radioactivity was recovered in the 105 000 g cell pellet, mainly in proteins. These experiments show that fatty acids containing photosensitive groups would potentially allow to localize the proteins involved in the binding and/or in the transport of fatty acids. |
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Keywords: | To whom all correspondence should be addressed. |
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