Determination of apoplastic K+ in intact leaves by ratio imaging of PBFI fluorescence |
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Authors: | Mhling, Karl Hermann Sattelmacher, Burkhard |
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Abstract: | The tetraammonium salt of the K+ binding fluorescent dye benzofuranisophthalate (PBFI) was used to investigate the influence ofpotassium nutrition (0.12.1 mol m3) on apoplasticK+ inVicia faba leaves by means of ratio imaging. As a referencethe infiltration-centrifugation method was used. Both methodsreflected the influence of K+ supply on apoplastic K+ concentration.The abaxial leaf side revealed significantly higher K+ concentrations(20-25 mol m3) than the adaxial side (58 mol m3).Application of CCCP led to an immediate increase in apoplasticK+ demonstrating the reliability of the PBFI method. Key words: Vicia faba, leaf, apoplast, K+, PBFI, ratio imaging, ratiometric fluorescence microscopy |
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