Cytophotometric Re-investigation of DNA content in Purkinje cells of the rat cerebellum |
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Authors: | V Mareš M van der Ploeg |
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Institution: | (1) Institute of Physiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia;(2) Department of Histochemistry and Cytochemistry, Sylvius Laboratories, Wassenaarseweg 72, NL-2333 AL Leiden, The Netherlands |
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Abstract: | Summary Stage scanning cytophotometry of Feulgen-stained cell nuclei of the cerebellum of young adult rats revealed that the absorbance values of the majority of the Purkinje cells show a Gaussian distribution with a low coefficient of variance. The peak absorbance of this population is the same as that of the granule cells. About 1% of the Purkinje cells measured, were found to have a stain content which indicates a 4C amount of DNA. For both the granular and the Purkinje cell population, a very small number of nuclei possesses absorbance values intermediate between 2C and 4C. The present data suggest prevalent diploidy of the Purkinje cells, and are at variance with those postulating a tetraploid and/or hyperdiploid status of this population. |
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