Enzymatic determination of oxalic acid-C14 in plant material |
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| Authors: | B L Bengtsson |
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| Affiliation: | 1. Graduate Program in Therapeutic Innovation (PPGIT), Bioscience Center. Federal University of Pernambuco, Av. Prof. Mores Rego, s/n, 50.670-420 Recife, PE, Brazil;2. Department of Pharmaceutical Science, Federal University of Pernambuco, Av. Artur de Sá, s/n, 50740-520 Recife, PE, Brazil;3. Pharmacognosy Laboratory - Federal University of Pernambuco (UFPE) Recife, Pernambuco 50670-901, Brazil;1. Laboratorio de Biotecnología e Inocuidad de los Alimentos. Municipalidad de Granadero Baigorria - Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR, Universidad Nacional de Rosario), Suipacha 531, S2000LRK Rosario, Argentina;2. Instituto de Química de Rosario (IQUIR, UNR-CONICET), Suipacha 570, S2000LRK Rosario, Argentina;3. Instituto de Biología de Rosario (IBR, UNR-CONICET), Suipacha 590, S2000LRK Rosario, Argentina |
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| Abstract: | A rapid and accurate manometric method for determining the amount and specific activity of total and water-soluble oxalic acid utilizing oxalic decarboxylase from Collybia velutipes (wood-rot fungus) is described. Two Warburg flasks were used for each determination; in one of them the gas exchange was measured, and in the other the liberated C14O2 was trapped on a paper moistened with 1 M Hyamine hydroxide in methanol. The paper was studied in a liquid scintillation counter with high counting efficiency. |
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