Insulinlike growth factor-1 (IGF-1)-induced stimulation of porcine preadipocyte replication |
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Authors: | J T Wright G J Hausman |
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Institution: | (1) R. B. Russell Research Center, Animal Physiology Research Unit, USDA, ARS, P.O. Box 5677, 30613 Athens, Georgia |
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Abstract: | Summary Insulinlike growth factor-1 (IGF-1) is both adipogenic and mitogenic to preadipose cell lines as well as primary stromal-vascular
(SV) cells. The precise effect of IGF-1 on primary preadipocytes per se, however, has not been elucidated directly. In this
study, primary porcine preadipocytes were exposed to IGF-1 while at three culture densities. The proportion of replicating
preadipocytes was determined by labeling cells with an antiadipocyte/preadipocyte monoclonal antibody (MAb) concomitant with
DNA measurement with propidium iodide. Flow cytometric analysis revealed that different seeding densities did not affect the
relative proportion of preadipocytes (AD-1 positive) in cultures. However, IGF-1 treatment increased the proportion of preadipocytes
at all densities but to a greater extent in more dense cultures. The resultant number of fat cell clusters formed was greater
at higher densities and on IGF-1 treatment. The proportion of replicating cells in cultures decreased with increasing density.
IGF-1 significantly increased replication at all densities and increased the number of replicating preadipocytes to the same
extent independent of density. These results provide direct evidence of hormonal regulation of primary preadipocyte replication.
Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA
and does not imply its approval to the exclusion of other products that may be suitable. |
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Keywords: | preadipocyte monoclonal antibody IGF-1 replication pig propidium iodide |
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