Application of immunoproteomics to rapid cytokine detection |
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Authors: | Boyle Michael D P Hess Jennifer L Nuara Anthony A Buller R Mark |
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Affiliation: | Department of Biology, Juniata College, Huntingdon, PA 16652, USA. boyle@juniata.edu |
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Abstract: | Cytokines are pivotal to a balanced innate or cell-mediated immune response, and can be indicative of disease progression and/or resolution. Methods to measure key cytokines rapidly with accuracy, precision, and sensitivity are consequently important. The current assay technologies, which are based on RT-PCR, immunoassays, or bioassays, are limited in their use in the clinic, in particular because of the long time (1-3 h) required to carry out the assays. An alternative, semi-quantitative approach described here, uses an immunological capture step and a mass spectral readout. The goal of the assay is speed rather than sensitivity or precision. |
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Keywords: | Animals Antibody specificity Antigen– antibody reactions Humans Immunoglobulin Fc fragments Interferon type II Protein array analysis Proteins Proteomics Serum albumin Isolation & purification Spectrometry, mass, matrix-assisted laser desorption-ionization Spectrum analysis, mass SELDI-TOF Cytokines Immunoproteomics Orthopoxvirus |
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